Axi-Core Chromatography Column
Axi-Core Chromatography Column
Introducton
The Axi-Core™ Automatic Chromatography Column is designed for high-efficiency separation and purification processes. This state-of-the-art column is engineered to meet the demanding needs of industrial and laboratory chromatography applications, ensuring consistent performance and reliable results. Axi-Core™ Columns are equipped with advanced axial compression technology, facilitating optimal packing of chromatography resins. Axi- Core™ columns are a link between kilo plant to manufacturing operations which handles hundreds of litres of various protein soups on various chromatography resins. Axi-Core™ column technology enables packing and purification operations with multiple variety of resins which are previous generations soft gels with single ligand chemistry or recent development of hardshell gels with single and/or multiple ligand chemistries.
The integration with a packing station ensures uniform resin distribution, reducing voids and channelling. Integration with packing station also enables to record a log using its own software which is 21 CFR part 11 complied. This handshaking with packing station needs less time, less requirements of number of persons and lesser requirement of total volume of resin while packing of any chromatography resins.
- Model Variants: Multiple sizes with SS316L or acrylic tubes.
- Materials: SS316L construction, optional SS904 mesh for chemical resistance.
- Automation: Automated resin packing, uniform slurry loading & axial compression.
Column Description
General
Axi-Core™ chromatography columns are new generation low-pressure enabling axial compression of loosely packed bed columns with the help of automated Axi-Core™ packing station protocols which don’t need any air operated diaphragm pumps and need not be connected to chromatography skids for the collection of column packing, unpacking data log recording. Intelligent alliance with self-operated Axi-Core™ packing station attributes hassle-free operations with less time and manual working hours, thereby increasing productivity.
Axi-Core™ columns come in various sizes like 450 mm diameter to 2000 mm diameter and with various acrylic and/or SS column tubes heights like 550mm, 600mm and 950 mm across all diameters. Productivity of operations depends on maintenance of columns. Across all diameters i.e. 450mm to 2000mm, there is “swing-out” mechanism of column tube across all diameters and tube heights available. This swing-out mechanism decreases maintenance time for replacing top or bottom adapter mesh and reduced time to minutes than hours of time.
| Main Components | Material | Brand Can be Chosen |
|---|---|---|
| Top Mesh | 316L | Sinter Filter Technik (China) / Bopp (Japan) |
| Bottom Mesh | 316L | Sinter Filter Technik (China) / Bopp (Japan) |
| Column Tube | Acrylic | Blanson (UK) |
| O-Ring | EPDM/FPM | Escort Seal (China) |
| Pull Rope Sensor | / | MIRAN (China) |
| Locking Cylinder | / | SMC (Korea) |
| Lead Screw Hoist | / | Torion (China) |
| Servo Motor | / | Delta Electronics (China) / Siemens (Germany) / Schneider (France) |
| Planetary Reducer | / | TRYXER (China) |
Axi-Core™ Master saves cost by assisting operations more than one column. Thus, it is not needed to purchase new Axi-Core™ Master for each column. Axi-Core™ Software enables creating folders in the inside memory for various columns at a time and which can be retrievable via using USB memory devices and/or can be stored as per data recording modified protocols set in the data management section.
Materials of Construction (MOC)
Axi-Core™ Chromatography column is widely used in various biosimilar molecules like vaccines, monoclonal antibodies, insulin and analogues and many more. Materials of construction used for Axi-Core™ columns satisfies ASME Bioprocessing Equipment standard, wetted polymeric materials meet requirements of USP VI as described in USP <88> Biological Reactivity Tests in vivo and 21 CFR Part 177 issued from FDA. Axi-Core™ column produces certificates required for free from any animal-derived components or in compliance with EMA/410/01 Rev.2.
Table 2 Axi-Core Column Specifications
| Axi-Core Column | 450 | 630 | 800 |
|---|---|---|---|
| Inner column diameter (mm) | 450 | 630 | 800 |
| Column type |
450X550 450X750 450X950
|
630X550 630X750 630X950
|
800X550 800X750 800X950
|
| Bed height (cm) | 420 620 820 |
410 610 810 |
400 600 800 |
| Weight, empty column4 (kg) | 420 460 500 |
900 950 1000 |
1800 1860 1920 |
| Min. height (mm) | 1600 1800 2000 |
1750 1950 2150 |
2550 2750 2950 |
| Max. operating work height (mm) | 2300 2500 2700 |
2400 2600 2800 |
2900 3100 3300 |
| Max. height for maintenance (mm) | 2500 2700 2900 |
2600 2800 3000 |
3100 3300 3500 |
| Max. operating pressure 4 bar (58 psi, 0.4 MPa) | 0.4 MPa 0.4 MPa 0.4 MPa |
0.4 MPa 0.4 MPa 0.4 MPa |
0.4 MPa 0.4 MPa 0.4 MPa |
| Footprint, foot (mm x mm) | 1050x1050 1050x1050 1050x1050 |
1200x1200 1200x1200 1200x1200 |
1500x1500 1500x1500 1500x1500 |
| Operating temperature (°C) 15–30 | 4–30°C 4–30°C 4–30°C |
4–30°C 4–30°C 4–30°C |
4–30°C 4–30°C 4–30°C |
| Adapter movement Servomotor | 1kW 1kW 1kW |
1.5kW 1.5kW 1.5kW |
1.5kW 1.5kW 1.5kW |
| Bed support (µm) | 10/20µm 10/20µm 10/20µm |
10/20µm 10/20µm 10/20µm |
10/20µm 10/20µm 10/20µm |
| Axi-Core Column | 1000 | 1200 | 1400 | 1600 | 2000 |
|---|---|---|---|---|---|
| Inner column diameter (mm) | 1000 | 1200 | 1400 | 1600 | 2000 |
| Column type | 1000x600 | 1200x650 | 1400x700 | 1600x700 | 2000x700 |
| Bed height (cm) | 450 | 500 | 550 | 550 | 550 |
| Weight, empty column (kg) | 3000 | 3500 | 4800 | 6000 | 7000 |
| Min. height (mm) | 2650 | 2000 | 2200 | 2600 | 2800 |
| Max. operating work height (mm) | 3100 | 2500 | 2750 | 3400 | 3600 |
| Max. height for maintenance (mm) | 3600 | 3200 | 3450 | 3600 | 3800 |
| Max. operating pressure 4 bar (58 psi, 0.4 MPa) | 0.35MPa | 0.35MPa | 0.32MPa | 0.3MPa | 0.3MPa |
| Footprint, foot (mm x mm) | 1730x1730 | 1950x1950 | 2100x2100 | 2350x2350 | 2800x2800 |
| Operating temperature (°C) 15–30 | 4–30°C | 4–30°C | 4–30°C | 4–30°C | 4–30°C |
| Adapter movement Servomotor | 2kW | 4kW | 4kW | 6kW | 9kW |
| Bed support (µm) | 10/20µm | 10/20µm | 10/20µm | 10/20µm | 10/20µm |
** Axi-Core columns are customizable as per client requirements